Composite

Part:BBa_K122001

Designed by: David Ouyang   Group: iGEM08_Rice_University   (2008-10-22)


[pGAL1][tetO][ZeoR]

This is a construct conferring zeocin (which belongs in the bleocin antibiotic family) resistance under the GAL1 promoter which is highly repressed by glucose. An additional tetracycline operator site was included upstream of the RBS to allow repression by tetR.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 361
    Illegal BamHI site found at 472
    Illegal XhoI site found at 860
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 757
    Illegal NgoMIV site found at 818
    Illegal AgeI site found at 89
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None